PDF(687 KB)
Research on the Lipid Constituents of Arisaema
Huang Zi-meng
Asia-Pacific Medical Frontiers ›› 2026, Vol. 1 ›› Issue (1) : 1-12.
PDF(687 KB)
PDF(687 KB)
Research on the Lipid Constituents of Arisaema
Objective The basic sources of A.erubescens in “Pharmacopoeia of the People's Republic of China” include Arisaema erubescens(wall.) Schott. Arisaema.heterophyllum Blume and Arisaema.amurense Maxim, which is most widely distributed in Liaoning, Sichuan and Yunnan provinces of China.The chemical constituents of A.erubescens are complex, but the reports of its main pharmacological and pharmacodynamic chemical constituents are not perfect; And it is impossible to identify the difference between A.erubescens from different sources. At present, there are few studies on the exclusive HPLC fingerprint of A.erubescens for identification and control of genuine and counterfeit products. Therefore, the separation and purification of lipid components in this paper is helpful to establish the exclusive HPLC fingerprint of A.erubescens and provide a basis for the identification of the quality of different basic A.erubescens and its decoction pieces. Methods:This method describes a complete process for the extraction and isolation of compounds from the tubers of *Arisaema heterophyllum*. First, the sliced medicinal material was subjected to reflux extraction with 95% ethanol, followed by concentration to obtain a total extract. The extract was then roughly separated by silica gel column chromatography using a gradient elution with dichloromethane-methanol and ethyl acetate-methanol solvent systems in varying ratios. The fractions were monitored by thin-layer chromatography (TLC), and those with similar compositions were combined to yield 11 fractions. Fraction III was further separated by Sephadex LH-20 gel column chromatography with 70% methanol as the eluent. The subfractions were monitored by polyamide TLC and combined to obtain four subfractions. Finally, after analysis by liquid chromatography, the target subfraction was purified using semi-preparative liquid chromatography to obtain a relatively pure sample. Results Two monomers were isolated and identified as terpineol and (E)-p-Coumaryl Alcohol by combining NMR and physicochemical properties. Conclusion:Pine alcohol and (E)-p-Coumaryl Alcohol were isolated from A.erubescens for the first time.It is suggested that the content of phenylpropanoids in fat-soluble components of A.erubescens may be higher.
A.erubescens / chemical sturcture / isolation / Phenylpropanoid
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