目的 本文运用网络药理学研究洋金花治疗银屑病的潜在作用机制。方法 本研究首先基于中药系统药理学分析平台联合PubChem化学信息学数据库实施洋金花药效成分高通量检索，继而运用Swiss Target Prediction靶标预测系统对候选活性化合物进行靶标谱系分析；同步通过OMIM疾病基因组学数据库与GeneCards基因注释知识库开展银屑病相关靶标系统捕获；最终借助Venny2.1.0生物信息学可视化工具实施药物-疾病靶标网络拓扑学分析，通过多维度验证机制揭示洋金花治疗银屑病的核心作用靶标群；利用STRING数据库构建蛋白质-蛋白质相互作用（PPI）网络，运用Cytoscape软件的筛选 PPI 核心基因；利用 Cytoscape 软件构建 “药物-成分-靶点-疾病”网络图；采用 DAVID 数据库对上述交集靶点进行基因本体和京都基因与基因组百科全书通路富集分析，获得药物治疗银屑病的生物学机制。结果 通过数据库的分析筛选出洋金花的靶点310个，获得银屑病相关靶点 4466个，共同靶点224 个；PPI网络筛选出关键靶点 49 个；GO 功能富集显示洋金花生物学过程和功能集中于蛋白质与三磷酸腺苷相关功能、细胞成分中的质膜与膜结构、分子功能中的蛋白质磷酸化和染色质重塑活性等；KEGG 通路富集显示，洋金花治疗银屑病主要涉及癌症中的信号通路（Pathways in cancer）、内分泌抵抗（Endocrine resistance）、糖尿病并发症中的AGE-RAGE信号通（AGE-RAGE signaling pathway in diabetic complications）、前列腺癌（Prostate cancer）等。 结论 本研究基于网络药理学研究方法发现，洋金花治疗银屑病的药效物质基础在于其含有的多活性成分可通过多靶点、多通路的协同调控网络发挥作用，该研究结果为进一步深入探究洋金花治疗银屑病的作用机制及临床应用提供了重要的理论支撑。

Objective This study aims to explore the potential mechanism of Datura stramonium in treating psoriasis using network pharmacology.Methods In this study, high-throughput retrieval of active components of Datura metel was first performed based on the Traditional Chinese Medicine Systems Pharmacology Analysis Platform combined with the PubChem chemoinformatics database. Subsequently, the Swiss Target Prediction system was used to conduct target spectrum analysis on candidate active compounds. Simultaneously, psoriasis-related targets were systematically captured through the OMIM disease genomics database and GeneCards gene annotation knowledge base. Finally, the Venny 2.1.0 bioinformatics visualization tool was employed for topological analysis of the drug-disease target network, and core action target groups of Datura metel in treating psoriasis were revealed through multi-dimensional validation mechanisms. The protein-protein interaction (PPI) network was constructed using the STRING database, and the core genes of PPI were screened plugin of Cytoscape software. The "drug-component-target-disease" network diagrams were constructed using Cytoscape software. The DAVID database was used to conduct gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis on the above intersection target sites to obtain the biological mechanism of Datura stramonium in treating psoriasis.Results Through database analysis, 310 target sites of Datura stramonium were screened out, 4466 psoriasis-related target sites were obtained, and 224 common target sites were identified. 49 key target sites were screened out from the PPI network. GO functional enrichment analysis shows that the biological processes and functions of Datura metel are concentrated on protein- and adenosine triphosphate (ATP)-related functions, plasma membrane and membrane structures in cellular components, and protein phosphorylation and chromatin remodeling activity in molecular functions, etc. KEGG pathway enrichment showed that the treatment of psoriasis by Datura stramonium mainly involved pathways in cancer, endocrine resistance, AGE-RAGE signaling pathway in diabetic complications, prostate cancer, etc.Conclusion Based on the network pharmacology research method, this study found that the pharmacological basis of Datura stramonium in treating psoriasis lies in its multiple active components that can exert effects through a multi-target and multi-pathway synergistic regulatory network. The research results provide important theoretical support for further in-depth exploration of the mechanism of Datura stramonium in treating psoriasis and its clinical application.