PDF(1340 KB)
PDF(1340 KB)
PDF(1340 KB)
桔梗不同部位水提物对小麦种子萌发的影响
Effects of aqueous extracts from different parts of Platycodon grandiflorus (Jacq.) A.DC. on wheat seed germination
目的 桔梗中含有大量的三萜皂苷、挥发油、黄酮、多糖和氨基酸等成分,对植物种子的萌发生长具有一定的影响。此次实验主要是采用培养皿滤纸法研究桔梗不同部位水提物对小麦种子萌发的影响。方法 实验先对桔梗不同部位进行水提,分别取2 g桔梗不同部位粉末,用100 mL超纯水在65 ℃下进行水浴提取1 h,得到母液,再稀释成适宜浓度提取液,然后制作成培养基来对小麦种子进行培养,通过观察空白对照组、桔梗不同部位水提物下的培养基上小麦种子的萌发状况,测算不同时段下各组的小麦种子发芽率和发芽势,来分析桔梗不同部位水提物对小麦种子萌发的影响。结果 桔梗根水提物、茎水提物对小麦种子发芽均有一定的促芽作用,而且茎提取物比根提取物的促芽效果更好,但在同一浓度下,桔梗叶片提取物对小麦种子萌发几乎呈现抑制作用。
Objective Platycodon grandiflorus (Jacq.) A.DC. contains a lot of triterpenoid saponins, volatile oil, flavonoids, polysaccharides and amino acids, which have some effects on the germination and growth of plant seeds.In this experiment, the effect of aqueous extracts from different parts of Platycodon grandiflorus (Jacq.) A.DC. on wheat seed germination was studied by using culture dish filter paper method. In the experiment, different parts of Platycodon grandiflorus (Jacq.) A.DC. were extracted by water, 2 g powder of different parts of Platycodon grandiflorus (Jacq.) A.DC. were extracted by water bath with 100 mL ultra-pure water at 65 °C for 1 h, and mother liquor was obtained, then diluted to the appropriate concentration of extract solution, then we made the medium to culture the wheat seeds, through observing the germination of the wheat seeds on the medium of the blank control group and the water extract of different parts of Platycodon grandiflorus (Jacq.) A.DC., to analyze the effect of aqueous extracts from different parts of Platycodon grandiflorus (Jacq.) A.DC. on the germination of wheat seeds, the germination percentage and germination potential of wheat seeds were measured at different time.The results showed that the aqueous extract from roots and stems of Platycodon grandiflorus (Jacq.) A.DC. had a certain effect on the germination of wheat seeds, and the effect of the aqueous extract from stems was better than that of roots, but at the same concentration, the aqueous extract of leaves of Platycodon grandiflorus (Jacq.) A.DC. showed almost inhibitory effect on wheat seed germination.
Platycodon grandiflorus (Jacq.) A.DC.
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