目的 研究甜叶菊根多糖（Stevia rebaudiana polysaccharideSRP）对非酒精性脂肪性肝病（NAFLD）的预防作用及其对细胞脂肪积累的治疗作用。方法 培养LO2细胞并测定其增殖活性，用不同浓度的甜叶菊根多糖SRP处理LO2细胞以确定甜叶菊对LO2细胞是否具有毒性，用油酸（OA）诱导LO2细胞，构建NAFLD细胞模型，使用cck8来测量细胞活性，并使用油红O染色来监测脂肪积聚。在不同浓度的甜菊多糖SRP中用油酸（OA）处理诱导的LO2细胞，并进行油红色O染色，观察甜菊多聚糖SRP对LO2脂肪积累的改善作用。结果 SRP处理后，SRP显著减少了LO2细胞周围脂肪的积累。结论 甜叶菊根多糖可以通过调节脂质代谢紊乱和减少细胞周围脂肪的积累来预防和治疗非酒精性脂肪肝。因此，甜叶菊根多糖在预防和治疗NAFLD中发挥着重要作用。

Objective To investigate the preventive effect of stevia rebaudiana polysaccharide (SRP) on non-alcoholic fatty liver disease (NAFLD) and its therapeutic effect on cell fat accumulation. Methods Cultivation of LO2 cells and measurement of their proliferative activity, treatment of LO2 cells with stevia rebaudiana root polysaccharides at different concentrations to determine whether stevia rebaudiana cells have toxicity to LO2 cells, induction of LO2 cells with oleic acid (OA), development of a cell model of NAFLD in vitro, Use of cck8 to measure cell activity, and with oil red O staining to observe fat accumulation.Treatment with oleic acid (OA) in different concentrations of Stevia Rebaudiana polysaccharide induced LO2 cells and oily red O staining was performed to observe the improvement of Stevia Rebaudiana polysaccharide on LO2 fat accumulation. Results After SRP treatment, SRP significantly reduced the accumulation of fat around LO2 cells. Conclusion Stevia rebaudiana polysaccharide can prevent non-alcoholic fatty liver disease (NAFLD) by regulating lipid metabolism disorders and reducing the accumulation of fat around cells.Therefore, stevia root polysaccharide plays an important role in the prevention and treatment of NAFLD.